Using GEFs to Deorphanize Odorant Receptors

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Using GEFs to deorphanize odorant receptors.

Odorant perception is initiated when odorants activate unique combinations of odorant receptors (ORs) expressed in the cilia of olfactory sensory neurons in the nose (Buck and Axel 1991; Malnic et al. 1999). One of the greatest challenges in the olfactory science is to correlate ORs with their ligands (to deorphanize the ORs). The determination of OR– ligand pairs should reveal how the OR famil...

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Tuning Insect Odorant Receptors

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The Question The first time I thought about olfaction was when I read a 1985 paper from Sol Snyder's group that discussed the unsolved question of how odors are detected in the nose (Pevsner et al., 1985). This paper opened up a fascinating new world for me. It was estimated that humans could perceive 10,000 or more chemicals as having distinct odors. Even more remarkably, subtle changes in an ...

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Insect Odorant Receptors: Channeling Scent

Odorant detection in insects involves heterodimers between an odorant receptor (OR) and a conserved seven-transmembrane protein called Or83b, but the exact mechanism of OR signal transduction is unclear. Two recent studies in Nature (Sato et al., 2008; Wicher et al., 2008) now reveal that these OR-Or83b heterodimers form odorant-gated ion channels, revealing a surprising new mode of olfactory t...

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G protein-coupled odorant receptors: From sequence to structure.

Odorant receptors (ORs) are the largest subfamily within class A G protein-coupled receptors (GPCRs). No experimental structural data of any OR is available to date and atomic-level insights are likely to be obtained by means of molecular modeling. In this article, we critically align sequences of ORs with those GPCRs for which a structure is available. Here, an alignment consistent with availa...

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ژورنال

عنوان ژورنال: Chemical Senses

سال: 2008

ISSN: 0379-864X,1464-3553

DOI: 10.1093/chemse/bjn058